Fastest human ever, 8-time Olympic gold medalist, and 11-time world champion has lost his last solo race to long time rival Justin Gatlin.
Bolt had announced the 16th IAAF World Championship in London would be his final competition.
Bolt finished third in 9.95 seconds, equalling his best time for 2017, behind Americans Gatlin who finished in 9.92 seconds, and Christian Coleman who clocked 9.94 seconds.
Gatlin, who has been banned twice for doping, was booed before the race, and after he won. He is winning what is his second world championship 12 years after the first.
Usain Bolt will retire next week after the 4×100 meters relay finals.